Studying at the University of Verona

Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.

A.A. 2019/2020

Academic calendar

The academic calendar shows the deadlines and scheduled events that are relevant to students, teaching and technical-administrative staff of the University. Public holidays and University closures are also indicated. The academic year normally begins on 1 October each year and ends on 30 September of the following year.

Academic calendar

Course calendar

The Academic Calendar sets out the degree programme lecture and exam timetables, as well as the relevant university closure dates..

Definition of lesson periods
Period From To
I semestre Oct 1, 2019 Jan 31, 2020
II semestre Mar 2, 2020 Jun 12, 2020
Exam sessions
Session From To
Sessione invernale d'esame Feb 3, 2020 Feb 28, 2020
Sessione estiva d'esame Jun 15, 2020 Jul 31, 2020
Sessione autunnale d'esame Sep 1, 2020 Sep 30, 2020
Holidays
Period From To
Festa di Ognissanti Nov 1, 2019 Nov 1, 2019
Festa dell'Immacolata Dec 8, 2019 Dec 8, 2019
Vacanze di Natale Dec 23, 2019 Jan 6, 2020
Vacanze di Pasqua Apr 10, 2020 Apr 14, 2020
Festa della Liberazione Apr 25, 2020 Apr 25, 2020
Festa del lavoro May 1, 2020 May 1, 2020
Festa del Santo Patrono May 21, 2020 May 21, 2020
Festa della Repubblica Jun 2, 2020 Jun 2, 2020
Vacanze estive Aug 10, 2020 Aug 23, 2020

Exam calendar

Exam dates and rounds are managed by the relevant Science and Engineering Teaching and Student Services Unit.
To view all the exam sessions available, please use the Exam dashboard on ESSE3.
If you forgot your login details or have problems logging in, please contact the relevant IT HelpDesk, or check the login details recovery web page.

Exam calendar

Should you have any doubts or questions, please check the Enrolment FAQs

Academic staff

B C F L P R V Z

Ballottari Matteo

matteo.ballottari@univr.it 045 802 7098

Bolzonella David

david.bolzonella@univr.it 045 802 7965

Boscaini Maurizio

maurizio.boscaini@univr.it

Bossi Alessandra Maria

alessandramaria.bossi@univr.it 045 802 7946 (Studio) - 045 802 7833 (Laboratorio)

Chignola Roberto

roberto.chignola@univr.it 045 802 7953

Fiammengo Roberto

roberto.fiammengo@univr.it 0458027038

Frison Nicola

nicola.frison@univr.it 045 802 7965

Furini Antonella

antonella.furini@univr.it 045 802 7950; Lab: 045 802 7043

Fusco Salvatore

salvatore.fusco@univr.it Office: +39 045 802 7954 Lab: +39 045 802 7086

Lampis Silvia

silvia.lampis@univr.it 045 802 7095

Perduca Massimiliano

massimiliano.perduca@univr.it +39 045 802 7984

Rossato Marzia

marzia.rossato@univr.it +39 045 802 7800
Foto personale,  July 18, 2012

Vallini Giovanni

giovanni.vallini@univr.it 045 802 7098; studio dottorandi: 045 802 7095

Vitulo Nicola

nicola.vitulo@univr.it 0458027982

Zaccone Claudio

claudio.zaccone@univr.it +39 045 8027864

Study Plan

The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University. Please select your Study Plan based on your enrolment year.

ModulesCreditsTAFSSD
1 module between the following
1 module among the following
6
B
(BIO/11)
1 module between the following
6
B
(ING-IND/25)
1 module between the following
ModulesCreditsTAFSSD
1 module between the following
Training
3
F
-
Final exam
36
E
-

1° Year

ModulesCreditsTAFSSD
1 module between the following
1 module among the following
6
B
(BIO/11)
1 module between the following
6
B
(ING-IND/25)
1 module between the following

2° Year

ModulesCreditsTAFSSD
1 module between the following
Training
3
F
-
Final exam
36
E
-
Modules Credits TAF SSD
Between the years: 1°- 2°
English B2 level
3
F
-
Between the years: 1°- 2°

Legend | Type of training activity (TTA)

TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.




SPlacements in companies, public or private institutions and professional associations

Teaching code

4S008292

Coordinatore

Salvatore Fusco

Credits

6

Scientific Disciplinary Sector (SSD)

BIO/10 - BIOCHEMISTRY

Language

Italian

Period

II semestre dal Mar 2, 2020 al Jun 12, 2020.

Learning outcomes

The course aims at providing students with advanced knowledge in the field of biocatalysis and new-frontiers application of these abilities to solve biotechnological problems as well as to design and synthesize new molecules.

Program

1. Introduction to the course and general information on biocatalysis: General information on the concepts of bio-resources and eco-sustainability; Main characteristics of enzymes; Cofactors and coenzymes; Nomenclature and classification of enzymes; Isoenzymes, multienzyme systems and complexes.

 2. Principles of thermodynamics and kinetics of chemical reactions: Bioenergetics and Thermodynamics; Activation energy and transition state theory; Kinetics of chemical reactions; Rate ​​and order of reactions; Collision theory and the concept of fruitful collisions (Arrhenius equation); Factors that influence the reaction rate.
 
3. Principles of enzyme catalysis and kinetics: Catalytic power and binding energy; Examples of enzyme catalysis; Determination and meaning of the initial velocity (v0); Michaelis-Menten equation; The meaning of the parameters of the M-M equation: Initial velocity (v0), maximum velocity (Vmax), constant of M-M (Km), number of turnover (kcat) and specificity constant (kcat / Km); Enzyme reactions with multiple substrates.

4. Mechanisms of reaction and regulation of the enzyme activity: Mechanism of reaction of chymotrypsin and application of pre-stationary state kinetics; Effect of pH on enzyme activity; Applications of the pH effect to study reaction mechanisms; Example of reaction mechanisms (aspartyl protease, hexokinase, enolase, lysozyme); Regulatory enzymes (allosteric enzymes), reversible covalent modifications and proteolysis (zymogens)

5. Linearizations of the M-M equation and enzymatic inhibition: Linear representations of the M-M equation: Lineweaver – Burk graphs (of the reciprocal doubles), Eadie – Hofstee and Hanes; Enzyme inhibition (reversible vs irreversible); Chemical agents that modify the enzymes irreversibly; Irreversible inhibitors: directed to the active site (Affinity Labels), suicide substrates (trojan horse) and Transition state analogues (Tight-Binding Inhibitors)

6. Effect of pH and temperature on enzymatic activity: pH dependence of reactions catalyzed by enzymes; Measuring the enzyme activity as a function of pH; Effect of pH on enzymatic activity in the presence of one or two ionizable groups; Dependence of kinetic constants as a function of pH; Van't Hoff and Arrhenius equations; How temperature-dependance activity and thermostability of enzymes are measured; The importance of the thermal stability for industrial applications.

7. Practical aspects of the study of enzymatic kinetics: Study of progression curves; Determination of initial velocity and enzyme units; Limitations on the measurement of the initial speed (dead time and stopped assays); Factors that influence the determination of enzyme activity (solvents, ionic strength, pH and temperature); Enzyme stability and storage methods.

8. Methods to measure enzyme activity (enzyme assays): UV/visible spectroscopy; spectrofluorimetry; Luminescence; Radioactivity; Direct and indirect enzyme assays; Coupled enzyme assays; Enzyme assays for diagnostics; Enzyme immunoassays; Continuous and discontinuous enzymatic assays.

9. Protein engineering and enzyme reactions in unconventional media: Protein engineering (principles and definitions); Chemical modifications; Genetic modifications: Rational design versus Directed evolution; Features of the screening methods;
-Rational design techniques: PCR with MegaPrimer, Whole plasmid PCR, Cassette mutagenesis, the Kunkel method, the QuikChange site-direct mutagenesis method;
- Directed evolution techniques: Error-Prone PCR, MEGAWHOP, Gene Assembly Mutagenesis, Mutator strains, random oligonucleotide-mediated mutagenesis, DNA Shuffling, DNA recombination by random priming, Staggered Extension Process, in vitro recombination methods (RACHITT, ITCHY, SCRATCHY, SHIPREC);
-Semi-rational design techniques: Structure-based combinatorial protein engineering (SCOPE) and SCHEMA structure-guided recombination;
- Enzyme reactions in unconventional mediums (medium engineering); enzymatic reactions in organic solvents; biphasic systems, co-solvents and pure organic solvents; other unconventional media (ionic liquids, supercritical fluids and eutectic mixtures).

10. Homogeneous and heterogeneous enzyme catalysis: Areas of application of homogeneous catalysis; Multienzyme systems (linear, parallel, orthogonal and cyclic enzyme cascades); Practical examples of enzyme cascades (production of perfumed chemical compounds, non-natural amino acids, plastic precursors, di-substituted pyrrolidine, D-phenylalanine derivatives); Heterogeneous catalysis (immobilised enzymes); Advantages and disadvantages of immobilisation; Supports for immobilisation; Immobilisation strategies (covalent, adsorption, entrapment and encapsulation); Carrier-less Immobilisation (CLECs and CLEAs); New frontiers of enzyme immobilisation: viral supports and nanoreactors.

11. Biocatalysis applied to plastics: Physicochemical properties that influence the degradability of plastics; Enzymes that hydrolyse plastic polymers (PET hydrolytic enzymes (PHEs)); Protein engineering to optimise PHEs; Ideonella sakaiensis as a model organism that metabolises PET plastic; Specific enzymes for the hydrolysis of PET (PETase); Lactic acid production from lignocellulosic biomass as a precursor to PLA bioplastics; use of lactic acid bacteria for the production of bioplastic; Pre-adaptation strategy to optimise lactic acid production.

12. The CRISPR-Cas system (principles and applications): CRISPR loci and cas genes; Mechanism of the CRISPR-Cas system; The three main types of CRISPR/Cas systems; Insights on the CRISPR/Cas9 system; Application of the CRISPR/Cas9 system for genome editing and protein engineering; Variants of the Cas9 endonuclease and their applications; the CRISPR/Cas9 system as a library screening tool for mutants generated by directed evolution.

13. Cytochromes P450, a family of promiscuous catalysts: Main features of cytochromes P450; The importance of catalytic promiscuity for the evolution of new enzymes; Types of reactions catalysed by cytochromes P450; Advantages and disadvantages of the use of P450 cytochromes; Main classes of cytochromes P450; The catalytic cycle of cytochromes P450; Uncoupling reactions; Engineering of cytochrome P450BM3 for hydroxylation of alkanes; Development of non-natural reactivity for the cycle-propanation of alkenes; The importance of axial ligand in cytochromes P450 for the development of non-natural reactivities.

During the course the lecturer will provide materials (book chapters, scientific articles and slides), which will be made available through the Moodle platform.

The topics shown are only indicative of the course's contents and may be subject to change by the teacher.

Bibliografia

Reference texts
Author Title Publishing house Year ISBN Notes
Hans Bisswanger Practical Enzymology Wiley-Blackwell 2011

Examination Methods

The final assessment aims to verify the achievement of the "Learning Outcomes" related to the topics reported in the "Course syllabus".
The final assessment consists of an oral dissertation aimed at ascertaining both that the student has acquired the knowledge defined in the course syllabus as well as the ability to perform the required logical-deductive links. In particular, the completeness of the exposition, the level of integration between the various course topics as well as the scientific appropriateness of the language will be evaluated.
Furthermore, the achievement by the student of a global vision of the topics addressed in class (combined with their critical application), the ability to make connections and the use of an appropriate scientific language will be assessed with a mark of excellence.
The final assessment follows the same guidelines for both attending and non-attending students.

Type D and Type F activities

I semestre From 10/1/19 To 1/31/20
years Modules TAF Teacher
Python programming language D Maurizio Boscaini (Coordinatore)

Career prospects


Module/Programme news

News for students

There you will find information, resources and services useful during your time at the University (Student’s exam record, your study plan on ESSE3, Distance Learning courses, university email account, office forms, administrative procedures, etc.). You can log into MyUnivr with your GIA login details.

Attendance

As stated in point 25 of the Teaching Regulations for the A.Y. 2021/2022, attendance is not mandatory. However, professors may require students to attend lectures for a minimum of hours in order to be able to take the module exam, in which case the methods that will be used to check attendance will be explained at the beginning of the module. 
Please refer to the Crisis Unit's latest updates for the mode of teaching.

Gestione carriere


Prova finale

La tesi di laurea può essere redatta e discussa dallo studente in lingua italiana o inglese. Nell'elaborato il laureando metterà in evidenza le esperienze acquisite ed il lavoro di ricerca sperimentale svolto su una tematica specifica presso laboratori di ricerca universitari, nazionali o internazionali, oppure presso qualificate istituzioni o enti pubblici o privati del settore biotecnologico, per un periodo non inferiore agli 8 mesi. L'elaborato sarà preparato dallo studente sotto la supervisione di un docente-relatore., eventualmente affiancato da uno o più correlatori. La Commissione di Valutazione è costituita da un Relatore e due Controrelatori. Può rivestire il ruolo di relatore di tesi ogni docente del Corso di Laurea, o altro docente afferente al Dipartimento di Biotecnologie, o altro docente dell’ Ateneo, in quest’ultimo caso previa autorizzazione del Collegio Didattico possono svolgere il ruolo di Correlatori i ricercatori operanti in Istituti di ricerca extrauniversitari, assegnisti di ricerca, titolari di borsa di studio post-dottorato, dottorandi di ricerca. I Controrelatori devono essere docenti dell'Area di Scienze ed Ingegneria. Sono nominati dalla Commissione Didattica almeno 25 giorni prima della discussione della tesi di laurea, verificata l’ammissibilità dello studente a sostenere la prova finale.

Valutazione della tesi di laurea
Per ogni presentazione sono a disposizione circa 30 minuti più la discussione. I criteri su cui è chiamata ad esprimersi la Commissione di Valutazione sono i seguenti: 1. livello di approfondimento del lavoro svolto, in relazione allo stato dell’arte dei settori disciplinari di pertinenza alle Biotecnologie Industriali; 2. avanzamento conoscitivo e/o tecnologico apportato dalla tesi; 3. impegno critico espresso dal laureando; 4. impegno sperimentale espresso dal laureando; 5. autonomia di lavoro espressa dal laureando; 6. significatività delle metodologie impiegate; 7. accuratezza nell’impostazione e nella stesura della tesi; 8. chiarezza espositiva. I Controrelatori non sono chiamati ad esprimersi sul punto 5.

I lavori della Commissione di Valutazione non sono regolati da convocazioni ufficiali e hanno luogo su accordo tra i soggetti interessati entro i 15 giorni precedenti la seduta di Laurea, nella quale si procederà alla sola proclamazione. Alla fine della presentazione, la Commissione di Valutazione stilerà una breve nota di valutazione con espressione di un voto sintetico (da 0 a 8). Questa nota sarà trasferita alla Segreteria di Corso di Laurea, entro il giorno precedente la seduta di laurea, per la successiva formulazione del voto definitivo da parte della Commissione di Laurea che procederà alla proclamazione.

Voto di laurea
Il voto di Laurea è espresso in centodecimi ed è un valore intero compreso tra 66/110 e 110/110. ll voto viene formato dalla somma, arrotondata al numero intero più vicino (es. 93.50 diventa 94 mentre 93.49 diventa 93), dei seguenti addendi: 1) media pesata sui crediti e rapportata a 110 dei voti conseguiti negli esami di profitto; 2) valutazione del colloquio di Laurea e della Tesi. Al colloquio di Laurea e alla Tesi sono attribuiti al massimo 11 punti. Essi saranno così distribuiti: a) 8 punti alla Commissione di Valutazione che valuterà il colloquio di Laurea e la Tesi secondo le seguenti modalità: attribuzione di un coefficiente compreso tra 0 e 1 (frazionario con una cifra decimale) per ciascuno dei punti 1-8 elencati sopra; b) 3 punti alla Commissione di Laurea che si esprime in modo assembleare. La commissione di Laurea attribuirà i punti in base alla valutazione del curriculum del laureando. In particolare: la presenza di eventuali lodi ottenute negli esami sostenuti, la partecipazione a stage ufficialmente riconosciuti dall’ ateneo, il superamento di esami in soprannumero ed il raggiungimento della Laurea in tempi contenuti rispetto alla durata normale del corso degli studi possono essere utilizzati dalla Commissione per l’attribuzione del punteggio. ll candidato che ottiene meno di 11 punti per la tesi può eventualmente avere un punto in più per la partecipazione ai programmi Socrates/Erasmus o affini, a discrezione della Commissione di laurea. c) somma del punteggio derivante da a) e b). Qualora la somma finale raggiunga 110/110, la Commissione di Laurea può decidere l’attribuzione della lode. Nel caso della proposta di laurea con lode lo studente deve avere una media ponderata minima di 104/110 (senza arrotondamenti) oppure una media ponderata minima di 102/110 (senza arrotondamenti) e aver conseguito almeno n. 3 lodi. In base alle norme vigenti, la lode viene attribuita solo se il parere della Commissione di Laurea è unanime.

Tesi di laurea esterne
Una tesi di laurea esterna viene svolta in collaborazione con un Ente diverso dall’ Università di Verona. In tal caso, il laureando dovrà preventivamente concordare il tema della tesi di laurea con un relatore del CdS LM8. E’ previsto almeno un Correlatore appartenente all’ente esterno, quale riferimento immediato per lo studente nel corso dello svolgimento della attività di tesi. Relatore e Correlatori devono essere indicati nella domanda di assegnazione della tesi di laurea. I risultati contenuti nella tesi di laurea sono patrimonio in comunione di tutte le persone ed Enti coinvolti.

List of theses and work experience proposals

theses proposals Research area
Valutazione dell'applicazione di nanoparticelle biogeniche per il controllo del cancro batterico del kiwi AGRICULTURE - AGRICULTURE
Valutazione dell'applicazione di nanoparticelle biogeniche per il controllo del cancro batterico del kiwi APPLICATIONS OF LIFE SCIENCES - APPLICATIONS OF LIFE SCIENCES
Valorizzazione di scarti agroindustriali mediante fermentazione termofila per la produzione di acidi organici come precursori chimici di polimeri Applied biotechnology (non-medical), bioreactors, applied microbiology - Applied biotechnology (non-medical), bioreactors, applied microbiology
Valutazione dell'applicazione di nanoparticelle biogeniche per il controllo del cancro batterico del kiwi Applied biotechnology (non-medical), bioreactors, applied microbiology - Applied biotechnology (non-medical), bioreactors, applied microbiology
Immobilizzazione di enzimi d’interesse industriale su nanoparticelle biomimetiche magnetiche Applied Life Sciences and Non-Medical Biotechnology: Applied plant and animal sciences; food sciences; forestry; industrial, environmental and non-medical biotechnologies, nanobiotechnology, bioengineering; synthetic and chemical biology; biomimetics; bioremediation - Biomimetics
Immobilizzazione di enzimi d’interesse industriale su nanoparticelle biomimetiche magnetiche Applied Life Sciences and Non-Medical Biotechnology: Applied plant and animal sciences; food sciences; forestry; industrial, environmental and non-medical biotechnologies, nanobiotechnology, bioengineering; synthetic and chemical biology; biomimetics; bioremediation - Non-medical biotechnology and genetic engineering (including transgenic organisms, recombinant proteins, biosensors, bioreactors, microbiology)
Immobilizzazione di enzimi d’interesse industriale su nanoparticelle biomimetiche magnetiche Chemical engineering, technical chemistry - Chemical engineering, technical chemistry
Effetto delle condizioni operative applicate al processo di digestione anaerobica su produzione di biogas e stabilità del carbonio organico del digestato Earth System Science: Physical geography, geology, geophysics, atmospheric sciences, oceanography, climatology, cryology, ecology, global environmental change, biogeochemical cycles, natural resources management - Biogeochemistry, biogeochemical cycles, environmental chemistry
Influenza dalla variazione stagionale del feedstock sulla produzione di biogas e sulla stabilità del carbonio organico presente nel digestato prodotto Earth System Science: Physical geography, geology, geophysics, atmospheric sciences, oceanography, climatology, cryology, ecology, global environmental change, biogeochemical cycles, natural resources management - Biogeochemistry, biogeochemical cycles, environmental chemistry
Frazionamento fisico e caratterizzazione dei pool di sostanza organica del suolo in vigneti Earth System Science: Physical geography, geology, geophysics, atmospheric sciences, oceanography, climatology, cryology, ecology, global environmental change, biogeochemical cycles, natural resources management - Sedimentology, soil science, palaeontology, earth evolution
Influenza del pascolamento sul ciclo biogeochimico di C ed N in una torbiera a sfagno Earth System Science: Physical geography, geology, geophysics, atmospheric sciences, oceanography, climatology, cryology, ecology, global environmental change, biogeochemical cycles, natural resources management - Sedimentology, soil science, palaeontology, earth evolution
Valorizzazione di scarti agroindustriali mediante fermentazione termofila per la produzione di acidi organici come precursori chimici di polimeri Environmental biotechnology, bioremediation, biodegradation - Environmental biotechnology, bioremediation, biodegradation
Bilanci di massa e di materia in digestori anaerobici alimentati con residui agricoli e zootecnici. Products and Processes Engineering: Product design, process design and control, construction methods, civil engineering, energy processes, material engineering - Chemical engineering, technical chemistry
Studio della composizione chimica e della stabilità termica di poliidrossialcanoati ottenuti da diverse matrici ambientali. Products and Processes Engineering: Product design, process design and control, construction methods, civil engineering, energy processes, material engineering - Chemical engineering, technical chemistry
Valorizzazione di scarti agroindustriali mediante fermentazione termofila per la produzione di acidi organici come precursori chimici di polimeri Products and Processes Engineering: Product design, process design and control, construction methods, civil engineering, energy processes, material engineering - Chemical engineering, technical chemistry
Immobilizzazione di enzimi d’interesse industriale su nanoparticelle biomimetiche magnetiche Products and Processes Engineering: Product design, process design and control, construction methods, civil engineering, energy processes, material engineering - Materials engineering (metals, ceramics, polymers, composites, etc.)
Valorizzazione di scarti agroindustriali mediante fermentazione termofila per la produzione di acidi organici come precursori chimici di polimeri Products and Processes Engineering: Product design, process design and control, construction methods, civil engineering, energy processes, material engineering - Production technology, process engineering
Studio delle proprietà di luminescenza di lantanidi in matrici proteiche Synthetic Chemistry and Materials: Materials synthesis, structure-properties relations, functional and advanced materials, molecular architecture, organic chemistry - Colloid chemistry
Multifunctional organic-inorganic hybrid nanomaterials for applications in Biotechnology and Green Chemistry Synthetic Chemistry and Materials: Materials synthesis, structure-properties relations, functional and advanced materials, molecular architecture, organic chemistry - New materials: oxides, alloys, composite, organic-inorganic hybrid, nanoparticles
Stampa 3D di nanocompositi polimerici luminescenti per applicazioni in Nanomedicina Synthetic Chemistry and Materials: Materials synthesis, structure-properties relations, functional and advanced materials, molecular architecture, organic chemistry - New materials: oxides, alloys, composite, organic-inorganic hybrid, nanoparticles

Further services

I servizi e le attività di orientamento sono pensati per fornire alle future matricole gli strumenti e le informazioni che consentano loro di compiere una scelta consapevole del corso di studi universitario.