Teaching code

4S004476

Teacher

Massimiliano Perduca

Coordinator

Massimiliano Perduca

Credits

6

Language

Italian

Scientific Disciplinary Sector (SSD)

BIO/11 - MOLECULAR BIOLOGY

Period

FARMACIA 2° semestre dal Jan 2, 2025 al May 30, 2025.

Courses Single

Not Authorized

Lessons timetable Moodle Seminars 0

Learning objectives

During the course the student will learn the basics of molecular biology which include:

-structure and topology of DNA and different classes of RNA
- Organization of DNA and RNA in the eukaryotic and prokaryotic cell
-Duplication, replication and transcription of DNA in prokaryotes and eukaryotes
- RNA: maturation and splicing
- From RNA to proteins: translation of RNA, genetic code, protein synthesis in prokaryotes and eukaryotes
- Introduction on the regulation of gene expression
- Overview on techniques of molecular biology

Prerequisites and basic notions

No prior knowledge is required.
A knowledge on Basic Biology is suggested.

Program

- Genetic information and informational molecules
General introduction and historical hints. The chemical structure of DNA and RNA. Three dimensional structure of DNA. Physico-chemical properties of DNA.
- DNA, RNA and gene structure
Definition of gene coding and regulatory regions. From genes to proteins; messenger RNA, transfer RNA and ribosomal RNA.
- Genome organization and evolution
DNA content and number of genes. Mutations, DNA rearrangement and genome evolution. The organelle genomes. Interrupted genes; introns. cDNA. Gene families and duplication. DNA repeats.
- Transposable elements
Transposition mechanisms and control. Retroviruses and retrotransposones. Transposons.
- Chromatin and chromosomes
Nucleosomes, histones and their modifications. Higher organization levels of chromatin. Heterochromatin and euchromatin. Eukaryotic chromosomes, telomeres and centromeres.
- DNA replication
DNA polymerases. Proofreading activity of DNA polymerases. Replication mechanism in bacteria and eukaryotic cells.
- Introns and RNA splicing
Features of spliceosomal introns. Spliceosome and splicing mechanism. Alternative splicing and trans-splicing. Other kinds of introns: group I and group II introns and tRNA introns. The intron movement. RNA editing. Ribozymes and riboswitch.
- DNA mutation and repair
Spontaneous mutations and mutations caused by physical and chemical mutagens. Pre- and post-replicative repair systems. Recombination in the immunity system cells. Approaches to homologous recombination.
- Regulation of gene expression
Bacterial promoters. The operon. Activators, repressors and coactivators. Signal transductions and two component regulation systems. Eukaryotic promoters. Activators, repressors and coactivators. Gene expression and chromatin modifications. Epigenetic mechanisms.
- RNAs and transcription
Different types of RNA: synthesis and maturation. Bacterial RNA polymerase. Sigma factors. Eukaryotic RNA polymerases. Eukaryotic mRNAs: capping, polyadenylation, cytoplasmic localization. The transcription process in bacteria and in eukaryotic cells.
- Translation
Ribosomes. tRNA structure and function. Aminoacyl-tRNA synthesis. Initiation in bacteria and eukaryotic cells. Polypeptide chain synthesis and translation end. Regulation of translation.
- Protein localization.

Bibliography

Didactic methods

The teacher will use frontal lessons and he will stimulate the interaction with students. Didactic material useful to this course will be uploaded on the Moodle page in pdf format.
Students unable "pro tempore" to attend classroom lessons will be able to download the pdf files related to the various topics from the Moodle page of the course.
The possibility of following the lessons in streaming will be granted only to those students with severe disabilities and absolutely unable to attend the classroom, through prior agreement with the teacher.

Learning assessment procedures

Oral examination.
The final exam will be on two questions concerning any of the topics treated during the course and it is passed if both answers are positive.

Evaluation criteria

The ability to discursively organize knowledge, the ability to reason critically on the study carried out, the quality of the exposition and the competence in the use of specialist vocabulary will be assessed.
after this time passed crystals were freeze-dried in liquid nitrogen.
The vote will be expressed out of thirty.

Criteria for the composition of the final grade

The grade will consist of the average of the assessment on the two questions.

Exam language

Italiano