Teaching code

4S00303

Teacher

Maria Romanelli

Coordinator

Maria Romanelli

Credits

6

Language

Italian

Scientific Disciplinary Sector (SSD)

BIO/13 - EXPERIMENTAL BIOLOGY

Period

1° semestre dal Oct 3, 2022 al Jan 20, 2023.

Location

VERONA

Lessons timetable Moodle Seminars 0

Learning objectives

The course aims to provide knowledge on cellular structures and functions, cell replication processes, transmission of genetic information, mechanisms of transcription and translation of the genetic information; general and up-to-date knowledge of biological issues that find application in the profession of sports activity experts; knowledge of modern biological investigation techniques that can be used in studies of motor activity and sports. Learning outcomes: understanding of the "scientific method" by describing and interpreting experiments that are part of the history of biology; description of the molecular processes that allow gene expression.

Prerequisites and basic notions

Being an exam of the first year, first semester, there are no specific prerequisites other than those required for access to the degree course.

Program

At the end of the course the student will learn about the following topics:
1. The characteristics common to all living organisms
2. The observations and experiments that led to the discovery of the genetic material: Griffith’s experiments and experiments by Avery, McLeod and McCarty on the identification of DNA; the central dogma of Biology, for which DNA derived from the RNA and protein by RNA; the discovery of the structure of DNA by Erwin Chargaff , Rosalind Franklin, J. Watson and F. Crick and finally to Meselson and Stahl (the semiconservative replication).
3 . The morphological and functional differences between viruses, bacteria and eukaryotes including protists.
4. The biology of the cell: the organization of the eukaryotic cell, the nuclear and cytoplasmic compartments. Microscopy and analytical techniques applied to the cell studies.
4.1 Cell membranes: the bilayer of phospholipids, cholesterol and membrane proteins, the relationship between the antigens on the erythrocyte membrane and blood groups. The transport mechanisms
4.2 The functions of the cell organelles.
4.3 The cell aggregates and the extracellular matrix
4.4 The morphology and function of the epithelial cells
4.5 The morphology of the muscle cell and the nerve cell, the neuromuscular junction and neurotransmitters, synapses, and its organization and the enzymes involved. Depolarization of the membrane, the chain of events that leads to the transmission of the signal from synapse to synapse through the axon, Schwann cells and oligodendrocytes; the action potential and muscle contraction. The muscle cell, molecules, enzymes and proteins involved in the contraction of the muscle fiber. The muscle fiber types and their characteristics, the growth of muscle mass related to training, the function of satellite cells, the function of myostatin and its inhibitors.
4.6 The cells of the connective tissue: diversity and functions
5. The cell reproduction: the cell cycle and the mechanisms that govern cell division (mitosis) and both male and female gametogenesis (meiosis). The stem cells.
6. The flow of gene expression: transcription and translation. The eukaryotic chromosome and gene regulation and expression. Chromatin and chromosomes. The human karyotype. Interpreting the switching on or off of the gene activity in response to an environmental stimulus; interpret the phenomena of differentiation and cell growth through mechanisms such as autocrine, paracrine and endocrine systems. Genetic mutations. The difference between gene mutations and chromosomal abnormalities, spontaneous mutations, mutagens and their action, the test of "fluctuation", the Ames’s test, the correlation between mutagenesis and cancer, the relationship between smoking and cancer, damage to UV and repair mechanisms, the Xerodema Pigmentosum.
7. Inheritance of characters: the principles of Mendelian genetics, the allels and the concepts of dominance and recessiveness.
8. Human genetics: interpreting and building family trees, with particular reference to characters such as the blood groups and some of the known genes in human genetic diseases (eg, familial hypercholesterolemia, cystic fibrosis, hemophilia, etc..)
9. The principles of population genetics population and the Hardy and Weinberg low.
10. The developmental biology: embryonal layers and their fate. The mechanisms involved in the aging; genetics and environmental factors; the Hayflick's experiments; the role of free radicals, antioxidants; the shortening of telomeres; the genetic programs that determine the average life span of a species.
The teaching methods consist of lectures during which the topics covered in the program will be discussed.
The assessment of learning outcomes envisages a written test to ensure knowledge of the topics under consideration.

Bibliography

Didactic methods

The teaching methods consist of classroom lessons during which the topics covered by the program will be addressed and discussed. The teacher will provide videos and power point presentations to supplement the classroom lessons, usable on the moodle platform. For students in isolation due to Covid positivity, registration or streaming will be made accessible on moodle. Students in particular situations of fragility, unable to attend lessons due to disability conditions or particularly disabling learning disorders, will be able to avail of similar support.

Learning assessment procedures

The assessment of the learning outcomes includes a written test aimed at ascertaining the knowledge of the topics in the program. The written test will include multiple choice questions and open-ended questions. To facilitate understanding of the contents and methods of examination, exam questions administered the previous year will be discussed in the classroom with the students of the following year.

Evaluation criteria

The written test potentially covers all the topics listed in the program. It is articulated in 30 questions related to the main themes of the course (life macromolecules, cell biology, cell interactions, genetic information flow, Mendelian inheritance, human genetics). Items are formulated as multiple answer questions and open answer questions. The answer to each item requires knowledge of biological terminology, the ability to interpret genealogy trees, the ability to systematically connect knowledge of biology and genetic molecules, ability to describe and exemplify biological structures.
The evaluation of the written test will be expressed out of thirty and will be considered positive if greater than or equal to 18/30.

Criteria for the composition of the final grade

The evaluation of the written test will be expressed out of thirty and will be considered positive if greater than or equal to 18/30.

Exam language

Italiano