Clinical practice (2nd year)
Scientific Disciplinary Sector (SSD)
MED/46 - BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE
At the end of the training activities in the three fundamental areas (Microbiology, Pathological Anatomy, Immunohematology and Transfusion), the student must be able to achieve specific training objectives:
1. Microbiology Area:
• prepare and use the main main cultivation areas;
• apply all the techniques related to the sowing of biological materials, to the isolation and identification of the germs and to the production of antibiograms;
• use the basic instrumentation (laminar flow hoods, thermostats, pipettes and micropipettes);
• apply Gram stain and know the Zielh Nielsen and Giemsa staining techniques;
• apply the techniques related to serological determinations and analytical techniques in microplate;
• know the procedures and applications related to agglutination tests, immunofluorescence and ELISA and PCR techniques.
2. Pathological Anatomy Area: Histopathology Sector ;
• recognizing and describing "non-compliant" samples;
• know of methods of sampling of the surgical material;
• knowledge of methods of processing the material and the paraffin embedding of the processed material;
• use the microtome;
• prepare sections of cryo-included material using the cryostat;
• apply standard colors (EE) and the most used special colors (Alcian Pas, Giemsa, Masson, Gomori, C.A.B., Perls);
• use the automated system for mounts and slides;
• use the laboratory equipment by applying the methods of control and maintenance and disinfestation;
• carry out quality checks before delivery of readings for reading under an optical microscope. Cytopathology Sector;
• recognize and describe vaginal and extravaginal "non-compliant" samples;
• prepare cytological preparations from biological liquids (urine, ascitic fluid, pleural fluid, liquor) using centrifugation and cytocentrifuge;
• set up cytological preparations from sputum and bronco-aspirates;
• know the methodology related to "liquid-based cytology";
• apply the smear and fixation methods on slide of cytological material;
• perform standard Papanicolaou staining and rapid staining of haematoxylin Eosin;
• assemble the cytological preparations.
3. Immunohaematology and Transfusion Area:
• acquire the standard techniques for the preparation of the 1st and 2nd level blood components and their conservation;
• recognize the validation methods of the blood components (legal examinations, interpretation of the results);
• acquire the analysis techniques used in pre-and post-transfusion investigations, in Newborn Hemolytic Disease, Acquired Hemolytic Disease and in any other specialized SIT sectors;
• recognize the methods for accepting the camps.
The final exam is composed of:
1. An evaluation of the training period carried out and the elaborate product, by the reference guide of the structure where the student carries out the internship, with a maximum score of 10 points.
2. A written test consisting of 40 multiple choice and open questions on specific topics of the program. The maximum score is 8 points (0.2 points per question).
3. An oral exam to ascertain the knowledge of the topics in the program and the ability of the student to apply analytical and argumentative skills, maximum score of 12 points.
4.The final vote is expressed in thirtieths and the year is exceeded if you reach 18/30.