Teaching code

4S000330

Credits

6

Coordinatore

Giovanni Gotte

Language

Italian

Moodle Seminars

The teaching is organized as follows:

Learning outcomes

The Integrated Course of Structural and Functional Sciences of the Biomolecules intends to provide the basic knowledge of Chemistry and Biochemistry. In particular: - Knowledge of the scientific method and possible practical effects in laboratories. - Knowledge of the main concepts of General and Organic Chemistry. - Further attention to the notions of Organic Chemistry preparatory to Biochemistry. - Knowledge on the structure-function relationships of the main classes of biological macromolecules and on metabolic regulation at the molecular level. - Knowledge about the interconnections existing between the different biochemical processes and the energy transformations connected to them. At the end of the course, the student / student must demonstrate to have acquired terminologies and notions useful for a critical analysis of chemical processes and vital biochemical phenomena in order to achieve autonomy of critical evaluation of the processes themselves, and appreciate their applicability in laboratory practice. MODULO CHIMICA E PROPEDEUTICA BIOCHIMICA The Chemistry course provides knowledge of: - The scientific method, underlined to explain phenomena occurring in the laboratories. - The main notions of General and Organic Chemistry. - Further attention is spent for notions, especially of Organic Chemistry, which are preparatory for the study of the Biochemistry. At the end of the Course, students should demonstrate to have acquired the proper scientific terms and notions in order to critically and autonomously evaluate the chemical processes and their application in the laboratory practice. MODULO BIOCHIMICA. The course is mostly focused on the relationship between structure and function of biological macromolecules and the main metabolic pathways in which they are involved. The fundamental objective is to stimulate the student's interest in biomolecular science by highlighting interconnections between biochemical processes and related energetics.At the end of the Course the student should demonstrate to have acquired the proper scientific terms and notions in order to critically and autonomously evaluate the biochemical processes of life.

Program

CHEMISTRY
-Matter: aggregation states, energy, intensive and extensive properties.
-Atom: atomic theory, atomic weight. Inside the atom: protons, neutrons, electrons. Orbital wave function. Quantum numbers and periodic properties. Connection between micro- and macroscopic levels: the mole.
-Chemical bond: ionic, covalent, weak bonds: hydrogen bond and hints of hydrophobic interactions. The ‘noble gas’ or octet rule.
-States of the matter: The law of ideal gases; real gases; vapour pressure; liquids and solutions; hints of solids.
-Chemical reactions: syntheses, swapping; acid-base and pH, redox reactions; examples of biochemistry redox reactions.
-Chemical equilibrium: in aqueous solution or not; the amphoteric nature of water. Connection between Keq and deltaG.
-Thermodynamics and kinetics: principles of thermodynamics and spontaneous, or not, reactions. Kinetic theory of collisions, activation energy and catalysis.
-Organic chemistry: the chemistry of carbon; hybridation of the carbon orbitals; stereochemistry; structure, nomenclature, functional groups of the oxygenated organic compounds, and reactivity of them. Aromatic compounds: structure and reactions. Nitrogen compounds and and most important reactions.

BIOCHEMISTRY
- Structure and function of proteins: amino acids; peptide bond; structural levels of proteins; fibrous proteins and globular proteins; hemoglobin and myoglobin; hemoglobin variants; classification, function and regulation of enzymes. Chromatography.
- Bioenergetics: metabolism, chemical transformations in the cell; thermodynamics; ATP as energy exchanger; oxidation/reductions.
- Structure and metabolism of carbohydrates; mono- and disaccharides; polysaccharides. Glycolysis and its regulation. Alcoholic and lactic fermentation.. Gluconeogenesis. Penthose phosphate pathway. structure, function and metabolism of glycogen.
- Oxidative decarboxylation of pyruvate: synthesis of AcetylCoA. Citric acid cycle; electron transport chain and oxidative phosphorylation; ATP synthesis.
- Structure and metabolism of lipids: structural lipids and biological membranes; lipids transport; lipids digestion and fatty acids beta-oxidation; hints on fatty acids biosynthesis
- Amino acid metabolism; transamination and oxidative deamination; urea cycle; metabolism of the carbon chain.

Frontal teaching is the exclusive method adopted in this Course.

Bibliography

Examination Methods

The exam modalities, unchanged with respect to the past ones, are reported here below. Certainly, the unpredictable conditions induced by the pandemic COVID-19 will probably induce some modifications that will be communicated promptly.

To pass the Chemistry module test, the students must demonstrate they acquired the comprehension and knowledge of the program topics. Furthermore, they must show the capability to expose their reasoning in a critical and precise manner, by using an appropriate scientific language.
The assessment of the subjects' learning in the Chemistry and Biochemistry modules is carried out through a joint oral test. The exam is preceded by a short written test consisting of 7 multiple-choice Chemistry Questions and 7 Biochemistry Multiple Questions. This test gives access to the oral exam only if the correct answers are at least five for each subject and remains valid for the entire academic year.
The final score will be expressed in thirtys (/30). The examination will be validated with a minimum score of 18/30.
6 examination appeals are foreseen in the whole Academic Year: 2 in the Winter Session, after the Course ending, 2 in the Summer Session, and 2 in the Autumn session.