Teaching code

4S003688

Credits

6

Coordinator

Daniela Cecconi

Language

English

Scientific Disciplinary Sector (SSD)

CHIM/01 - ANALYTICAL CHEMISTRY

Courses Single

Authorized

Moodle Seminars 0

The teaching is organized as follows:

Learning objectives

Module: theory The aim of clinical proteomics is to detect molecular markers useful in the clinical setting. In particular, the purpose of clinical proteomics is to identify dysregulated pathways and protein biomarkers that can be useful for diagnosis, prognosis, and prediction of therapeutic outcomes, as well as to clarify pathogenic mechanisms. Upon completing this course, students will have the necessary knowledge to recognize strengthens and weakness of the different proteomics methodologies, as well as to recognise their application to current areas of clinical investigation. Module: laboratory The student will acquire laboratory skills through a series of experiments concerning the proteomic analysis of biological samples by two-dimensional electrophoresis.

Prerequisites and basic notions

Being a first year, first semester exam, there are no specific prerequisites other than those required for access to the degree course.

Program

MM: Theory
• Introduction to clinical proteomics
• Strategies for protein sample preparation
• Gel-based and gel-free clinical proteomics analyses
• Protein Identification by Tandem Mass Spectrometry
• Label-Based and Label-free MS clinical proteomic approaches
• Differential profiling of Breast Cancer plasma proteome for biomarkers identification
• Clinical Proteomics to study Pancreatic Cancer Stem Cells
• Brain tissue proteomic analysis to identify biomarkers of Alzheimer’s Disease
• Evaluation of therapeutic effects of neural stem cells therapy in Parkinson’s disease
• Proteomics of cerebrospinal fluid to identify biomarkers for amyotrophic lateral sclerosis
• Pharmacoproteomics for elucidating the mechanism of action of anticancer drugs
MM: Laboratory
The student will participate to practical experiences which are typical analyzes of a clinical proteomics laboratory, such as: preparation of a protein extract for proteomic analysis, separation by isoelectric-focusing of proteins, separation by two-dimensional electrophoresis of protein extracts, staining and evaluation of proteomic profiles.

Bibliography

Didactic methods

Frontal teaching as the main teaching method.
In special cases, indicated by University guidelines, and by the request of individual students, recorded lessons may be made available.

Learning assessment procedures

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UL: teoria
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Participation in at least 30% of the lessons is recommended.
The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives.
The exam consists of a 2-hour written test. The exam method is NOT differentiated between attending and non-attending students and / or for Erasmus students and the course does NOT include intermediate tests.
The assessment test is semi-structured, as an open-ended questionnaire. The student will have to answer a series of open-ended questions, demonstrating that they understand and be able to use the fundamental concepts of each topic. The understanding of the practical experiences will be verified through questions included in the test relating to the theoretical course.
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UL: laboratorio
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Understanding of practical experiences will be verified through questions included in the test of the theoretical course.
The final vote is obtained from the following formula: Vote = Vote_written exam

Evaluation criteria

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UL: teoria
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The ability to discursively organize knowledge is assessed, as well as the deepening of knowledge, the ability to critically reason on the study carried out, the quality of the exposition, and the competence in the use of the specialized vocabulary.
Mark in thirtieths (max 30/30).
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UL: laboratorio
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The ability to discursively organize knowledge is evaluated, as well as the deepening of knowledge, the ability to critically analyze and evaluate the studied topics, the quality of the exposition, and the competence in the use of the specialized vocabulary.
Mark, expressed in thirtieths (max 30/30).

Criteria for the composition of the final grade

The final mark is obtained from the following formula: Mark = Mark_written exam

Exam language

------------------------ UL: teoria ------------------------ italiano e/o inglese ------------------------ UL: laboratorio ------------------------ english and/or italian