Studying at the University of Verona

Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.

This information is intended exclusively for students already enrolled in this course.
If you are a new student interested in enrolling, you can find information about the course of study on the course page:

Laurea in Biotecnologie - Enrollment from 2025/2026

The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.

2° Year  activated in the A.Y. 2014/2015

ModulesCreditsTAFSSD
6
B
BIO/18

3° Year  activated in the A.Y. 2015/2016

ModulesCreditsTAFSSD
Biofisica
6
A
-
Bioinformatica e banche dati biologiche
6
B
-
12
C
BIO/04 ,BIO/09
Fondamenti di processi e impianti biotecnologici
6
B
-
Tecnologie biomolecolari
12
B
-
Prova finale
3
E
-
activated in the A.Y. 2014/2015
ModulesCreditsTAFSSD
6
B
BIO/18
activated in the A.Y. 2015/2016
ModulesCreditsTAFSSD
Biofisica
6
A
-
Bioinformatica e banche dati biologiche
6
B
-
12
C
BIO/04 ,BIO/09
Fondamenti di processi e impianti biotecnologici
6
B
-
Tecnologie biomolecolari
12
B
-
Prova finale
3
E
-

Legend | Type of training activity (TTA)

TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.




S Placements in companies, public or private institutions and professional associations

Teaching code

4S00800

Credits

12

Language

Italian

Scientific Disciplinary Sector (SSD)

BIO/11 - MOLECULAR BIOLOGY

The teaching is organized as follows:

teoria

Credits

9

Period

II sem.

Academic staff

Massimiliano Perduca

laboratorio [1° turno]

Credits

3

Period

II sem.

Academic staff

Barbara Molesini

laboratorio [2° turno]

Credits

3

Period

II sem.

Academic staff

Barbara Molesini

Learning outcomes

The aim of this course is to give the students the basic knowledge of the molecular mechanisms concerning transmission, variation and expression of the genetic information.

Program

Theory:
-> Genetic information and informational molecules
General introduction and historical hints. The chemical structure of DNA and RNA. Three dimensional structure of DNA. Physico-chemical properties of DNA.
-> Molecular Biology techniques
Agarose gel electrophoresis. Nucleic acid hybridization. Polymerase chain reaction (PCR). Restriction endonucleases. Cloning and sub-cloning. gene expression systems.
-> DNA, RNA and gene structure
Definition of gene coding and regulatory regions. From genes to proteins; messenger RNA, transfer RNA and ribosomal RNA.
-> Genome organization and evolution
DNA content and number of genes. Mutations, DNA rearrangement and genome evolution. The organelle genomes. Interrupted genes; introns. cDNA. Gene families and duplication. DNA repeats.
-> Transposable elements
Transposition mechanisms and control. Retroviruses and retrotransposones. Transposons.
-> Chromatin and chromosomes
Nucleosomes, histones and their modifications. Higher organization levels of chromatin. Heterochromatin and euchromatin. Eukaryotic chromosomes, telomeres and centromeres.
-> DNA replication
DNA polymerases. Proofreading activity of DNA polymerases. Replication mechanism in bacteria and eukaryotic cells.
-> Introns and RNA splicing
Features of spliceosomal introns. Spliceosome and splicing mechanism. Alternative splicing and trans-splicing. Other kinds of introns: group I and group II introns and tRNA introns. The intron movement. RNA editing. Ribozymes and riboswitch.
-> DNA mutation and repair
Spontaneous mutations and mutations caused by physical and chemical mutagens. Pre- and post-replicative repair systems. Recombination in the immunity system cells. Approaches to homologous recombination.
-> Regulation of gene expression
Bacterial promoters. The operon. Activators, repressors and coactivators. Signal transductions and two component regulation systems. Eukaryotic promoters. Activators, repressors and coactivators. Gene expression and chromatin modifications. Epigenetic mechanisms.
-> RNAs and transcription
Different types of RNA: synthesis and maturation. Bacterial RNA polymerase. Sigma factors. Eukaryotic RNA polymerases. Eukaryotic mRNAs: capping, polyadenylation, cytoplasmic localization. The transcription process in bacteria and in eukaryotic cells.
-> Translation
Ribosomes. tRNA structure and function. Aminoacyl-tRNA synthesis. Initiation in bacteria and eukaryotic cells. Polypeptide chain synthesis and translation end. Regulation of translation.
-> Protein localization.

One credit of the course (corresponding to 8 hours) will be kept for the students to discuss an important topic chosen from the research literature in Molecular Biology.

Introduction to the Laboratory Course:
-> Nucleic acids isolation: basis, comparison of several extraction protocols, nucleic acids isolation troubleshooting.
-> Nucleic acids electrophoresis: agarose gels, polyacrylamide gels, denaturing and non-denaturing gels, Pulsed-field gel electrophoresis.
-> Spectrophotometric quantitation of isolated nucleic acids.
-> PCR
1.What is PCR?
2. Reagents: efficiency, specificity, fidelity
3. PCR cycle. Final number of copies of the target sequence
4.Amplifying the correct product: detection and analysis of PCR products, how to avoid contamination (uracil N-glycosylase, UV, enzymatic treatment), hot start, nested PCR
5. Techniques and applications: 5’RACE-PCR and 3’RACE-PCR, RT-PCR, PCR mutagenesis (deletion of sequences, base substitutions, insertion mutagenesis), modification of PCR products (introduction of restriction sites, adding promoters and ribosome-binding sites), joining overlapping PCR products, quantitative PCR

Experiments:
Total RNA extraction from different plant tissues using methods based on acid guanidinium thiocyanate-phenol-chloroform and adsorption to silica-gel membranes, DNase treatment, qualitative and quantitative evaluation of isolated total RNA samples employing electrophoresis on microfabricated-chips, first strand cDNA synthesis, semiquantitative Reverse transcription polymerase chain reaction (RT-PCR) and Quantitative Real-Time PCR utilizing SYBR Green chemistry. Rapid Amplification of cDNA Ends: 5'RACE-PCR; 3'RACE-PCR.

Bibliography

Reference texts
Activity Author Title Publishing house Year ISBN Notes
teoria Nancy L. Craig, Orna Cohen-Fix, Rachel Green, Carol W. Greider, Gisela Storz, Cynthia Wolberger Biologia molecolare Principi di funzionamento del genoma (Edizione 1) Pearson 2013 9788871928111
teoria Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick Lewin's Genes X (Edizione 10) Jones & Bartlett Publishers 2009 0763766321
teoria Harvey Lodish, Chris A. Kaiser, Anthony Bretscher, Angelika Amon, Arnold Berk, Monty Krieger, Hidde Ploegh and Matthew P. Scott Molecular Cell Biology (Edizione 7) Freeman 2012 1464102325
teoria Alberts et al. The Cell (Edizione 5) Garland Science 2007 978-0-8153-4105-5
teoria Geoffrey M. Cooper, Robert E. Hausman The cell: a molecular approach (Edizione 6) Sinauer Associates, Inc 2013 978-1-60535-155-1

Examination Methods

Oral examination preceded by a propaedeutic written exam concerning the Laboratory Course.

Students with disabilities or specific learning disorders (SLD), who intend to request the adaptation of the exam, must follow the instructions given HERE

Teaching materials e documents