Studying at the University of Verona
Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.
Study Plan
This information is intended exclusively for students already enrolled in this course.If you are a new student interested in enrolling, you can find information about the course of study on the course page:
Laurea in Biotecnologie - Enrollment from 2025/2026The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.
1° Year
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2° Year activated in the A.Y. 2014/2015
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3° Year activated in the A.Y. 2015/2016
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Legend | Type of training activity (TTA)
TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.
Plant molecular methods (2015/2016)
Teaching code
4S003257
Credits
6
Language
Italian
Scientific Disciplinary Sector (SSD)
BIO/04 - PLANT PHYSIOLOGY
The teaching is organized as follows:
teoria
laboratorio
Learning outcomes
At the end of the course, the student will acquire the technical skills for the design of various types of genetic constructs aiming at the development of transgenic, cisgenic, and intragenic plants. In addition, genome editing tools will be covered.
Particular attention will be paid to the application of these methods for crop improvement.
Supplied educational material:
Power point lessons, relevant research articles and reviews.
Program
Molecular mechanism of stable transformation via Agrobacterium; transgene integration, stability, methylation and silencing; strategies to avoid transgene silencing (e.g. site-specific recombination for precise and clean transgene integration); promoters used for genetic constructs (constitutive, spatiotemporal, inducible, synthetic); coupling synthetic promoters with synthetic transcription factors for coordinated expression of multiple genes; multigene engineering; reporter genes; artificial miRNA and target mimicry; cisgenesis and intragenesis; cisgenic and intragenic genetic constructs; strategies to remove marker genes from transgenic plants; artificial programmable DNA nucleases (ZFNs and TALENs) and RNA-guided DNA nucleases (Type II CRISPR-Cas9 of Streptococcus pyogenes) for genome engineering; molecular mechanism of CRISPR-Cas9; sgRNA design; minimization of off-target activity (e.g. Cas9 nickase and double nicking strategy and alteration in the length of the sgRNA); application of CRISPR-Cas system beyond genome editing (e.g. CRISPR interference, gene regulation, and cargo delivery); genetic constructs for genome engineering using the CRISPR-Cas9 system, screening of mutants generated by CRISPR system (e.g. RFLP analysis and T7 endonuclease I assay); hybridization between nucleic acids and DNA/RNA labelling.
Lab. experiences:
Histochemical localization of Mtp::GUS expression during Medicago truncatula root development: Composite plants generation using Agrobacterium rhizogenes; analysis of the transgenic state of adventitious roots and GUS staining.
Design of sgRNAs using free bioinformatic softwares, efficient production of sgRNAs and efficacy testing of sgRNAs for the cleavage in vitro of their targets in the presence of Cas9.
Examination Methods
written exam
