Teaching code

4S00097

Credits

6

Coordinator

Adolfo Speghini

Language

Italian

Scientific Disciplinary Sector (SSD)

CHIM/02 - PHYSICAL CHEMISTRY

Moodle Seminars 0

The teaching is organized as follows:

Learning outcomes

THEORY: the Course aims to provide the students with the basic tools for understanding and interpreting chemico-physical phenomena concerning systems of biological and biotechnological interest, also through the use of theoretical models. The student will acquire the ability to apply chemical-physical concepts to real processes in order to quantify observables, of thermodynamic, transport, kinetic and spectroscopic type. Some example cases on various physical-chemical aspects will be considered in order to familiarize the student with solution of real problems. LABORATORY: the course also includes some laboratory experiences to provide manual skills and critical skills in dealing with real chemico-physical problems, as well as providing knowledge on modern methods and equipment for the measurement of thermodynamic variables, kinetic constants, colloidal properties as well as for studying electronic and vibrational properties of molecules.

Program

THEORY

Thermodynamics.
Perfect and real gases. Concepts of heat and work. Heat capacity.
Internal energy, enthalpy and their variations with temperature.
Enthalpy of phase transition. Enthalpy of reaction and its variation with temperature.
Entropy and its variation with temperature. Entropy of phase transition. Overview on the statistical interpretation of entropy. Entropy of reaction.
Gibbs free energy and its variation with pressure and temperature. Stability condition and phase diagrams. Definition of chemical potential. Chemical potential of components of gas mixtures. Entropy and free energy of mixing for gaseous mixtures.
Chemical potential of components od ideal and real solutions. Free energy of mixing for ideal fluids.
Free energy of reaction. Equilibrium and equilibrium constant. Variation of the equilibrium constant with the temperature.

Chemical kinetics.
The rate of reactions. Rate law and order of reaction. Arrhenius equation. Transition state and activation energy. Determination of kinetic laws.
Approach to equilibrium and relaxation. Reaction mechanism. Consecutive reactions. Steady state approximation. Pre-equilibrium. Rate Determining Step. Chemical reactions controlled by diffusion or activation. Kinetic and thermodynamic control of a chemical reaction.

Atomic and molecular energy structure. Molecular spectroscopies.
Introduction to quantum theory. Particles in confined systems. Harmonic oscillator and molecular vibrational modes. Atomic structure. Hydrogen-like atoms. Spin. Pauli exclusion principle.
Valence bond and molecular orbitals. Electronic spectroscopy in the UV and visible regions. Circular dichroism. Decay of the excited states. Radiative and non-radiative transitions. Fluorescence and quenching. Phosphorescence. Vibrational spetroscopy.
Principles of Nuclear Magnetic Resonance spectroscopy (NMR).
Basics on Boltzmann distribution.

Colloids.
Colloidal dispersions and their stability. Examples of colloids of biological importance. Overview of nanostructured systems.
Hydrodynamic diameter and Zeta potential for colloids and their measurements with Dynamic Light Scattering (DLS) technique.


LABORATORY EXPERIENCES

1) Evaluation of the thermal capacity of a calorimeter and neutralization reaction enthalpy through calorimetric measurements;

2) Determination of the kinetic parameters for the reaction of hydrogen peroxide with iodine ion in acidic solution.

3) Study of spectroscopic transitions of fluorescine in the visible region through measurement and analysis of absorption and fluorescence spectra; fluorescence quenching of fluorescein with iodide ion; investigation on the quenching mechanism;

4) FTIR spectrometer and interpretation of infrared spectra of simple molecules. NMR spectrometer and interpretation of NMR spectra of simple molecules. DLS instrumentation for measuring the hydrodynamic diameter and Zeta potential of colloids.

Bibliography

Examination Methods

The oral examination will include all the topics of the Course about the theoretical part as well as the examples, exercises and laboratory experiences. Particular attention will be devoted on the Physical Chemistry concepts and the knowledge of the methods, tools and techniques used in laboratory experiences.
For both attending and not attending students the oral examination will cover all the topics discussed in the theoretical part, in the examples and exercises as well as in the laboratory experiences.
Written reports about the the laboratory experiences are required, describing the principles, the used experimental methods and the results obtained during the lab experiences. The reports have to be loaded to the Moodle platform as soon as the lab experiences will be completed.