Studying at the University of Verona
Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.
Study Plan
This information is intended exclusively for students already enrolled in this course.If you are a new student interested in enrolling, you can find information about the course of study on the course page:
Laurea magistrale in Biotecnologie agro-alimentari - Enrollment from 2025/2026The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.
1° Year
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Un insegnamento a scelta tra i seguenti:2° Year activated in the A.Y. 2012/2013
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Un insegnamento a scelta tra i seguenti:| Modules | Credits | TAF | SSD |
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Un insegnamento a scelta tra i seguenti:Legend | Type of training activity (TTA)
TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.
Molecular analytical methods - TRASCRITTOMICA (2011/2012)
Teaching code
4S02774
Credits
4
Coordinator
Not yet assigned
Language
Italian
Scientific Disciplinary Sector (SSD)
CHIM/01 - ANALYTICAL CHEMISTRY
To show the organization of the course that includes this module, follow this link: Course organization
The teaching is organized as follows:
Teoria
Laboratorio
Learning outcomes
This course introduces to some new analytical technologies widely used for genome analysis, functional genomics and trascriptomics. The purpose is to provide both knowledge of theory for these technologies as well as practical experience.
Program
Syllabus:
THEORY:
GENOMICS:
-GATEWAY TECHNOLOGY: Lamba phage recombination and introduction to gateway technology, BP and LR reactions, ccdB based selection, ENTRY clone production, TOPO cloning, gateway compatible destination vectors.
-MUTAGENESIS: DNA mutations, mutagenesis in forward and inverse genetics, random mutagenesis (chemical or PCR based), site directed mutagenesis (with or without PCR), Quickchange, DNA shuffling
TRANSCRIPTOMICS:
-RT-PCR AND REAL TIME RT-PCR: single gene expression analysis and genome-wide expression analysis, RT-PCR, Real Time RT-PCR, absolute and relative quantification (delta delta Ct method for data analysis), SYBR green analysis, TaqMan probes, LUX, Molecular Beacons and Scorpions
-MICROARRAY: cDNA microarray and their production, oligoarray and oligoarray production, direct and indirect probe labeling, experimental design and controls for microarray experiment setup, hybridization, image acquisition and data analysis
PRACTICAL PART:
Gene Cloning using the gateway technology and transfer of a gene in a destination vector
Expression analysis using real time RT-PCR
Microarray expression analysis
Examination Methods
Exam methods:
See main page of the integrated course
