Studying at the University of Verona
Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.
Study Plan
This information is intended exclusively for students already enrolled in this course.If you are a new student interested in enrolling, you can find information about the course of study on the course page:
Laurea magistrale in Biotecnologie agro-alimentari - Enrollment from 2025/2026The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.
1° Year
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Un insegnamento a scelta tra i seguenti2° Year activated in the A.Y. 2014/2015
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Un insegnamento a scelta tra i seguenti| Modules | Credits | TAF | SSD |
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Un insegnamento a scelta tra i seguenti| Modules | Credits | TAF | SSD |
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Un insegnamento a scelta tra i seguentiLegend | Type of training activity (TTA)
TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.
Molecular analytical methods - PROTEOMICA (2013/2014)
Teaching code
4S02774
Credits
4
Coordinator
Not yet assigned
Language
Italian
Scientific Disciplinary Sector (SSD)
CHIM/01 - ANALYTICAL CHEMISTRY
To show the organization of the course that includes this module, follow this link: Course organization
The teaching is organized as follows:
teoria
Credits
3
Period
II semestre
Academic staff
Daniela Cecconi
laboratorio
Learning outcomes
Proteomics is the large-scale study of proteins. It is an emerging scientific research which can be applied to food (to find allergen for example) and plants research (for example to study plant pathogen response).
Program
PROTEOMICS
Analytical proteomics (LM9+LM7):
1. Introduction.
2. Sample preparation.
3. Gel-based separation.
4. 2D gel Image analysis.
5. Gel-free separation.
6. Pre-fractionation of low abundance proteins.
7. Analysis of proteins/peptides by Mass Spectrometry.
8. Analysis of proteins/peptides by Tandem Mass Spectrometry.
9. Analysis of mass spectra and database search for protein identification.
10. Differential and Quantitative proteomics.
11. Characterization of post-translational modifications.
12. Proteomics in agro-food and biomedical areas.
Laboratory (1 CFU)
Day 1:
Rehydration of 7 cm IPG with protein extract
Preparation of buffers and solutions for 2-DE analysis
Polymerization of 12% T gel
Isoelectrofocusing by using PROTEAN® IEF Cell (Bio-Rad)
Day 2:
IPG strip equilibration
Interfacing with the second dimension
Second dimension by Mini-PROTEAN® 3 Dodeca™ Cell (Bio-Rad)
Staining of 2-DE maps with Coomassie and/or SyproRuby
Day 3:
Destaining and acquisition of images by VersaDoc™ MP 1000 system (Bio-Rad)
Image analysis by PDQuest software (Bio-Rad) (classroom gamma)
Analysis of mass spectra and database searching with Mascot
Examination Methods
written
Mandatory attendance to laboratory.
