Studying at the University of Verona

Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.

The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.

2° Year  activated in the A.Y. 2011/2012

ModulesCreditsTAFSSD
12
B
INF/01
12
C
BIO/10
6
C
BIO/18
activated in the A.Y. 2011/2012
ModulesCreditsTAFSSD
12
B
INF/01
12
C
BIO/10
6
C
BIO/18

Legend | Type of training activity (TTA)

TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.




S Placements in companies, public or private institutions and professional associations

Teaching code

4S01911

Credits

6

Coordinator

Stefano Capaldi

Language

Italian

Scientific Disciplinary Sector (SSD)

BIO/11 - MOLECULAR BIOLOGY

The teaching is organized as follows:

Laboratorio

Credits

4

Period

I semestre

Academic staff

Stefano Capaldi

Teoria

Credits

2

Period

I semestre

Academic staff

Stefano Capaldi

Learning outcomes

The aim of this couse is to provide a description of the basic techniques of recombinant DNA. In particular, the course focuses on the manipulation of nucleic acids, restriction endonuclease digestion, electrophoretic separation of DNA, and cloning. This topics are covered in the theoretical part of the course and in the laboratory as well.

Program

Theoretical part:
Recombinant DNA. Prockaryotic and eukaryotic vectors. Gene cloning. Genomic and cDNA libraries.
Nucleic acid manipulation. Restriction enzyme digestion. Electrophoresis. DNA ligation.
Polymerase Chain Reaction (PCR). Applications.
Bacterial coltures. Trasformation of bacteria and eukaryotic cells.
Recombinant protein production from cloned genes.

Laboratory:
Plasmid DNA purification
Genomic DNA purification
RNA purification
Electrophoresis and DNA purification from gel
Restriction endonuclease digestion
PCR and ligation
Recombinant protein expression and SDS PAGE and western blot analysis.

Examination Methods

Practical course report and oral examination

Students with disabilities or specific learning disorders (SLD), who intend to request the adaptation of the exam, must follow the instructions given HERE