Teaching code

4S000351

Credits

8

Coordinator

Martina Montagnana

Language

Italian

Moodle Seminars 0

The teaching is organized as follows:

Learning outcomes

MODULO BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE The course aims to provide students with the principles for knowledge: of specific terminology; direct and indirect analysis techniques for air, water and soil pollutants; the mechanisms, causes, effects and costs of pollution; purification techniques and self-purification phenomena; of techniques to limit pollution and its effects. MODULO BIOLOGIA MOLECOLARE The course also aims to provide an updated view of molecular biology techniques that are used in different clinical settings including prenatal diagnosis of genetic and chromosomal disorders, diagnosis of mendelian and oncoematologic diseases, infertility and pharmacogenetics. MODULO BIOTECNOLOGIE RICOMBINANTI The course aims to make students understand the basic stages of the birth of modern biotechnologies and the current applications of recombinant biotechnology in biomedical and pharmaceutical fields. We will discuss issues of a scientific, ethical and biological nature. The course also aims to provide an updated view of recombinant techniques including techniques for reproductive and therapeutic cloning, technic for the reproduction of recombinant drugs MODULO METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE The training objective is to provide adequate knowledge of the main diseases related to alterations of genes and theoretical principles of the techniques of cytogenetics and molecular genetics. Obtain adequate knowledge of the theoretical principles and applications of the techniques of detection of genetic alterations MODULO METODOLOGIA DIAGNOSTICA MOLECOLARE The course aims to make students understand the role of genetic testing in the clinical course of the patient. The course also aims to provide students who have no medical training the basic knowledge on the clinical aspects concerning the genetic basis of diseases. The course aims to provide an updated view of molecular biology techniques that are used in a clinical setting.

Program

------------------------ MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE ------------------------ Definitions; types of pollution; the causes of pollution; direct and indirect effects of pollution; methods of analysis; damage to health; costs; phenomena of self-purification; purification techniques; specific aspects of air, water and soil polluttants; landfills; incineration; composting. ------------------------ MM: METODOLOGIA DIAGNOSTICA MOLECOLARE ------------------------ Introduction and organization of the molecular biology laboratory. Clinical molecular biology in hematological field. CBC and peripheral blood smear. Main methods of proteomics (chromatography, spettrometry..). FISH and Array-CGH. ------------------------ MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE ------------------------ Organization of the human genome, genetic Polymorphisms, germ-line and somatic mutations. Online Databases for genomics. Reference search and genome browsers. In silico molecular biology software. Nucleic acids extraction. Quantification, quality analysis and labeling of nucleic acids. Gene amplification techniques (cloning), signal amplification, target amplification. Endpoint PCR and real-time qPCR. Analysis of real-time qPCR data. RT-qPCR and other gene expression assays. Digital PCR and new methods for multiplex nucleic acids analysis. Next generation sequencing( NGS), Array CGH, MLPA PCR, RNA fusion sequencing to detect chromosomal aberrations, SNPArray and RNAseq for gene expression analysis. Introduction to third-generation sequencing. Examples of biomedical applications of molecular methodologies. (companion Diagnostics, forensic Genetics, Pharmacogenetics). ------------------------ MM: BIOTECNOLOGIE RICOMBINANTI ------------------------ Lesson 1 A Short History of DNA Technology and the Human Genome Project Lesson 2 Major classes of restriction endonucleases, molecular cloning using a plasmid vector, different cloning vector Lesson 3 The laboratory animal species most closely related to humans for which the knockout technique can easily be applied, Knockout mouse. Lesson 4 Bioinformatic tools: BLAST and Primer 3 Lesson 5 Clinical applications of recombinant drugs. Human recombinant TSH. production technique, commercial products, pharmacokinetic properties, approval for clinical use. Use in the diagnosis and follow-up of differentiated thyroid carcinoma  ------------------------ MM: BIOLOGIA MOLECOLARE ------------------------ •Introduction and organization of the molecular biology laboratory •Cystic fibrosis: pathogenesis, clinical manifestations and diagnosis •Male infertility: laboratory tests in the diagnosis of congenital causes •Molecular biology in oncohematological diseases •Prenatal diagnosis: molecular and cytogenetic testing •Diagnostics of Genetic diseases in mental retardation •Hemocromatosis: pathogenesis, clinical manifestations and diagnosis •Application of Pharmaco-genetics and -genomics

Bibliography

Examination Methods

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MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
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Oral Examination
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MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
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Oral test. The exam is based on all the course contents. The exam is passed if the evaluation is greater or equal to 18/30.
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MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
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Oral Exam Should the emergency regulation persist in the next months, exam procedures will be modified according to the guidelines produced by the University.
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MM: BIOTECNOLOGIE RICOMBINANTI
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Oral exam
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MM: BIOLOGIA MOLECOLARE
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oral exam