Teaching code

4S003719

Credits

6

Coordinator

Not yet assigned

Language

Italian

Scientific Disciplinary Sector (SSD)

AGR/07 - AGRICULTURAL GENETICS

Moodle Seminars 0

To show the organization of the course that includes this module, follow this link:  Course organization

The teaching is organized as follows:

Learning outcomes

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MM: Metabolomica teoria
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- Define the “targeted” and “untargeted” metabolomics approaches; -discriminate the critical points of an experimental design; -organize a plan for the extraction and analysis of metabolites suitable for the metabolites/class of metabolites of interest; -describe the key elements for the raw data process till the production of the FQM (Feature Quantification Matrix); -use statistical approaches suitable to the experimental problem; -apply the metabolomics analysis to the investigation of the biological complexity of cells/ tissues/ organs/organisms, from the point of view of the small molecules, as intermediates or end products of the metabolism; -use an appropriate scientific language.
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MM: Trascrittomica laboratorio
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The laboratory part of this Course has as main aim to provide to students the knowledge for the correct design, application and interpretation of a transcriptomic analysis. Students will have the opportunity to approach and discuss the experimental design for a transcriptomic experiment, to deal with preparation of samples for RNASeq analysis and will apply bioinformatics tools to interpret transcriptomic data.
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MM: Trascrittomica teoria
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The theory part of this course aims to explain what a transcriptomic analyses is and for which purpose it can be applied. Furthermore it aims to provide knowledge about the different approaches and technique which can be applied for a transcriptomic analysis. By the end of the course students will be able to approach planning of transcriptomic analysis and to understand transcriptomic data and their interpretation presented in scientific paper.

Program

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MM: Metabolomica teoria
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-the experimental design; -metabolite extraction and preparation for LC-MS; -data formats and conversion; -chromatogram analysis and metabolite annotation; -data preprocessing; the effect of the technique of data normalization; -data processing (with MZmine): m/z feature extraction, chromatogram deconvolution and alignement; production of the Feature Quantification Matrix; -data analysis through multivariate analysis (PCA, PLS-DA, OPLS-DA) and univariate analysis.
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MM: Trascrittomica laboratorio
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MM: Trascrittomica teoria
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Examination Methods

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MM: Metabolomica teoria
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The examination will be an oral test aimed to verify : -the overall degree of knowledge of the subject of this course; -the scientific language skills.
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MM: Trascrittomica laboratorio
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The exam will be oral aiming to ascertain the students’ knowledge on the topics of lectures and lab practices. One third of the colloquium will be dedicated to test knowledge on topics treated in the lab part of the course. Student will discuss experimental planning or applications of transcriptomic analysis or apply tools for transcriptomic data analysis faced during the lab part. The exam will be same for both attending and non-attending students.
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MM: Trascrittomica teoria
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The exam will be oral aiming to ascertain the students’ knowledge on the topics of lectures and lab practices. Two third of the colloquium will be dedicated to test knowledge on topics treated in the theory part of the course. The exam will be same for both attending and non-attending students.