Teaching code

4S000331

Teacher

Maria Carla Tafi

Credits

1

Scientific Disciplinary Sector (SSD)

MED/46 - BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE

Language

Italian

Period

TEC LAB BIOM LEZ 1A 2S dal Feb 27, 2017 al Apr 28, 2017.

Moodle Seminars

To show the organization of the course that includes this module, follow this link:  Course organization

Learning outcomes

The approach to security in the microbiology lab (containment levels with related facilities and activities). Bio-booths.

Principles and techniques of sterilization by dry heat and moist, sterilization by filtration, radiation sterilization, gas sterilization.
Microscopic examination of bacteria: preparation of slides, staining for bacteria, fungi, protozoa, direct observation or fresh.Cultivation of bacteria: culture media: basic, enriched, selective, differential, enrichment, transport, for susceptibility testing, for fungi.
. Principles for identification of bacteria: identification based on the task biochemistry, identification based on serological techniques and immunological, microbial counting methods, methods for determining the sensitivity to antimicrobial agents: methods for dilution and for dissemination.

Culture and virus scanning: cultivation methods, highlighting of infected cells, quantitative assessment. Virus reactions to physical and chemical agents.

Program

The program approach to security in the microbiology lab (containment levels with related facilities and activities. The biosecurity cabins).
Principles and techniques of sterilization:
sterilization by heat dry and wet sterilization, by filtration sterilization, by radiation sterilization, by gas sterilization.
Techniques observation prepared by light microscopy:
optical microscope observation with lens dry, observation with immersion objective, observation brightfield, observation darkfield, observation in phase contrast .
Electron microscopy Principles
Microscopic examination of bacteria:
direct observation or fresh stains for bacteria, fungi, protozoa Cultivation of bacteria: Culture media: basal media, enriched, selective, differential, enrichment, transport, to antibiograms ,for fungi.
Cultivation of bacteria:
Culture media: basal media, enriched, selective, differential, enrichment, transport, to antibiograms for fungi.
Techniques and principles of sowing and isolation and identification of microorganisms:
the microbial isolation methods: general rules for isolation of the plate, the general isolation techniques, transfer of pure cultures, technical maintenance of pure cultures. Seeding techniques of biological samples.
Bacteria identification principles: identification based on the task biochemical identification based on serological and immunological techniques Methods of microbial counting methods for determining the sensitivity to antimicrobial agents: dilution and diffusion method
Techniques for the visualization of the antigen-antibody reaction:
enzyme immunoassay, immunofluorescence, latex agglutination test Notes on cultivation techniques in virology: cell cultures assessment of the growth of the virus in cell culture titration of virusess
Outline of molecular biology techniques applied to microbiological diagnostics:
Wester blot, Southern blot, PCR, qPCR.
Hints on quality control in microbiology.

Examination Methods