Studying at the University of Verona
Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.
Study Plan
This information is intended exclusively for students already enrolled in this course.If you are a new student interested in enrolling, you can find information about the course of study on the course page:
Laurea in Biotecnologie - Enrollment from 2025/2026The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.
1° Year
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2° Year activated in the A.Y. 2015/2016
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3° Year activated in the A.Y. 2016/2017
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Un insegnamento a scelta
Un insegnamento a scelta
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Un insegnamento a scelta
Un insegnamento a scelta
Legend | Type of training activity (TTA)
TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.
Methods in Microbiology and Genetics (2016/2017)
Teaching code
4S003254
Credits
12
Language
Italian
Scientific Disciplinary Sector (SSD)
AGR/07 - AGRICULTURAL GENETICS
The teaching is organized as follows:
laboratorio Metodologie di Genetica
teoria Metodologie di Genetica
Laboratorio Metodologie di Microbiologia [1° turno]
Laboratorio Metodologie di Microbiologia [2° turno]
teoria Metodologie di Microbiologia
Learning outcomes
The objective of the course of Methods in Genetics is to provide to the students the knowledge about the DNA recombinant technology and the methods used for the genomic and molecular genetics analyses. Lectures will offer an overview of the most common and innovative genetic methodologies applied in the analysis of genes and their function. The acquired knowledge will allow students to understand the experimental part of scientific paper in genetic field.
The course of Methods of Microbiology aims to provide students with the tools of knowledge in the sectors of microbiology and biotechnology to enable them to deal with the higher level courses of biotechnology; in particular it helps to understand the potential applications of microorganisms in the agri-food field and the interaction among microorganisms, food, intestinal tract and health.
The objective of practical training is to gain the skill to operate in a laboratory of microbiology by applying traditional and biomolecular approaches
Program
Methods in Genetics: The course describes the basic methods used in genetic analysis and/or molecular biology for DNA and RNA handling. Program includes the following topics: Vectors and selection markers, most common cloning techniques, GATEWAY and TOPOCLONING. Plasmid and genomic DNA extraction. Methods for bacterial transformation. Selection of recombinant colonies. Utilization of reporter genes (GFP, YFP) for cellular localization. Animal and plant cell cultures. Animal and plant cells genetic transformation. Protoplast preparation and transfection (Prof. Furini)
Methods for genomic DNA extraction. Genome analysis by Southern blotting, molecular markers based on hybridization or PCR. Linkage analysis. Sanger DNA sequencing and introduction to NGS sequencing. Random and site specific mutagenesis and application for forward and reverse genetics. Introduction to genome editing. RNA extraction and gene expression analysis through Northern blotting, RT-PCR and Real Time RT-PCR (Prof. Bellin)
Methods of Microbiology: There are 2 CFU of exercises during which traditional and biomolecular approaches will apply to:
- the study of bacterial cultures used in the production of functional foods (eg. probiotic fermented milks).
- the detection and characterization of commensal bacteria with antibiotic resistance in products with complex microbiota,
- the assessment of the effect of various yeasts, including a genetically modified strain of Saccharomyces cerevisiae, on the fermentation kinetics and production of metabolites of interest for the quality of wine in microvinification trials (Prof. Torriani)
Examination Methods
Written examination based on lectures and practical laboratory lessons.