Learning objectives

The course aims to guide students to understand both the various applications of CRISPR-Cas technology in proteomics and the uses of proteomics in different fields to confirm gene mutations and monitor the impact of gene editing on the total proteome. Additionally, the hands-on practical lesson aims to guide students with the ability to use the main bioinformatics tools, which allow the interpretation of proteomic datasets to identify the phenotypic effects of CRISPR-Cas9 editing.

Prerequisites and basic notions

Basic concepts of proteomic analysis.

Program

Theoretical learning course:
• Applications CRISPR-Cas to proteomics for: studying protein-protein interactions, analysing protein-chromatin interactions and generation of cellular models
• Proteomics applied for confirming gene mutation by CRISPR-Cas9 at the protein level and quantification of the target product
• Proteomics applied for monitoring of changes in the total proteome, thus exploring the overall impact of gene editing on the protein expression
• Functionality-based bioinformatics to enable interpretation of large-scale proteomics data providing biologically meaningful results about the phenotypic effects of CRISPR-Cas9 editing.

Practical learning course “Hands-on lesson”:
• Practical bioinformatics lesson using the main web-based bioinformatics tools (e.g., Cytoscape, WebGestalt, PANTHER, DAVID, STRING) to analyse the effect of CRISPR-Cas9 editing on the proteomic phenotype. In particular, Gene Ontology enrichment analysis of the differentially expressed proteins, Functional enrichment analysis to detect dysregulated pathways and Protein-protein interaction network analysis will be performed.

Didactic methods

Frontal lectures

Learning assessment procedures

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Assessment

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Scheduled Lessons