Teaching code

4S003264

Credits

6

Coordinator

Daniela Cecconi

Language

Italian

Scientific Disciplinary Sector (SSD)

CHIM/01 - ANALYTICAL CHEMISTRY

Courses Single

Authorized with reserve

Moodle Seminars 0

The teaching is organized as follows:

Learning objectives

Theory The course aims to provide students with the basic principles for the interpretation of analytical data and the reliability of analytical measurements (errors, preanalytical and analytical variability, precision, accuracy, sensitivity and specificity, prediction). The most important analytical techniques for clinical chemistry will be described (such as techniques for the enzymatic determination of analyte concentration, immunochemical techniques, radiochemical and electrochemical techniques, immunoelectrophoresis). Upon completing the course, students will have the knowledge necessary to recognize the strengthens and weakness of the different techniques and their applicability to different biological samples. Laboratory The student will acquire laboratory skills through a series of exercises concerning the determination of enzymes and analytes in biological matrices such as serum and plasma.

Prerequisites and basic notions

Prerequisites as indicated in the Didactic Regulations: Biochemistry and Analytical Biochemistry.

Program

MM: theory
• Collection of biological materials.
• The components of the variability in clinical chemistry (pre-analytical, biological, analytical). Experimental errors.
• Precision, accuracy, sensitivity and specificity of analytical measurements.
• Predictability of an analytical test. Quality control of the results.
• Comparison of analytical methods (regression analysis, Bland-Altman plot).
• Techniques for the detection of enzyme activity. Enzymatic determination of analyte concentration.
• Immunochemical techniques. Heterogeneous (competitive / non-competitive), homogeneous (competitive / non-competitive).
• Immunoenzymatic assays (Enzyme Linked Immunosorbent Assay; Microparticle enzyme immunoassay; Enzyme Multiplied immunoassay; Cloned enzyme donor immunoassay).
• Immunofluorescent techniques (Immunofluorescence Assay, Fluorescence Polarization immunoassay; Dissociation-Enhanced Lanthanide Fluorescent immunoassay; IF Microscopy and FACS).
• Chemiluminescence (Lumino immunoassay; Chemiluminescent Magnetic immunoassay; ElectroChemiLuminiscence; Bioluminescence enzyme immunoassay; Immunoassays with avidin-biotin)
• Radioimmunoassay (Radio immunoassay; Immuno Radiometric Assay).
• Agglutination test. Complement fixation test.
• Immunodiffusion; Immunoelectrophoresis; Counter current electrophoresis.
Immunonephelometry and Immunoturbidimetry.
• Electrolytes and electrochemical techniques in diagnostics.
• Biosensors
MM: laboratory
The student will participate to practical experiences concerning the typical analyses of a clinical chemistry laboratory, for example glucose determination by GOD-POD enzymatic colorimetric method; determination of alkaline phosphatase by kinetic method DEA optimized; alanine aminotransferase determination by kinetic UV method IFCC optimized. The laboratory also include practical experiences concerning assays to determine transferrin, complement factor C3c and fibrinogen by radial immunodiffusion as well as analysis of immunodetection by enhanced chemiluminescence.

Bibliography

Didactic methods

Frontal teaching as the main teaching method.
Lessons will be delivered exclusively in presence.
Participation in at least 40% of the lessons is recommended.
REQUIREMENT TO ACCESS THE EXAMINATION: In order to take the final written exam, a scientific paper relating to one of the techniques described during the course must be presented in the classroom (Journal Club).
NON-attending students who intend to take the exam must contact the teacher within the first two weeks from the beginning of the course to be included in the calendar of exhibitions and to have assigned the scientific article.

Learning assessment procedures

The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives.
The exam consists of a 2-hour written test. The exam method is NOT differentiated between attending and non-attending students and / or for Erasmus students and the course does NOT include intermediate tests.
The assessment test is semi-structured, as a questionnaire with open answers. The student will have to answer a series of open-ended questions, demonstrating that they understand and be able to use the fundamental concepts of each topic. The understanding of the practical experiences will be verified through questions included in the test relating to the theoretical course.

Evaluation criteria

The ability to discursively organize knowledge is evaluated, as well as the deepening of knowledge, the ability to critically reason on the study carried out, the quality of the exposition, and the competence in the use of the specialized vocabulary.
Mark, expressed in thirtieths (max 30/30).

Criteria for the composition of the final grade

The final mark is obtained from the following formula: Mark = Exam_ Mark + max of 2 points for paper exposure

Exam language

italiano