Studying at the University of Verona
Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.
Study Plan
The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.
1° Year
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2° Year activated in the A.Y. 2018/2019
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3° Year activated in the A.Y. 2019/2020
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Legend | Type of training activity (TTA)
TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.
Diagnostic methodologies for Biochemistry and Molecular Biology - METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE (2019/2020)
Teaching code
4S000351
Teacher
Credits
2
Language
Italian
Scientific Disciplinary Sector (SSD)
MED/46 - BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE
Period
Lezioni TLB 1 SEMESTRE dal Oct 1, 2019 al Dec 20, 2019.
To show the organization of the course that includes this module, follow this link: Course organization
Learning outcomes
The training objective is to provide adequate knowledge of the theoretical principles and application of the techniques for detection of genetic alterations, in a functional framework considering the main diseases related to alterations of genes, including cancer.
Program
Organization of the human genome, genetic Polymorphisms, germ-line and somatic mutations.
Online Databases for genomics. Reference search and genome browsers. In silico molecular biology software.
Nucleic acids extraction. Quantification, quality analysis and labeling of nucleic acids.
Gene amplification techniques (cloning), signal amplification, target amplification. Endpoint PCR and real-time qPCR. Analysis of real-time qPCR data. RT-qPCR and other gene expression assays. Digital PCR and new methods for multiplex nucleic acids analysis.
Next generation sequencing( NGS), Array CGH, MLPA PCR, RNA fusion sequencing to detect chromosomal aberrations, SNPArray and RNAseq for gene expression analysis. Introduction to third-generation sequencing.
Examples of biomedical applications of molecular methodologies. (companion Diagnostics, forensic Genetics, Pharmacogenetics).
| Author | Title | Publishing house | Year | ISBN | Notes |
|---|---|---|---|---|---|
| Greg Gibson, Spencer V. Muse | A primer of Genome Science (Edizione 3) | Sinauer | 2009 | 978-0-87893-236-8 | |
| Terry A. Brown | Biotecnologie molecolari (Edizione 2) | Zanichelli | 2017 | 978-88-08-32096-4 | |
| Mauro Maccarrone | Metodologie Biochimiche e biomolecolari (Edizione 1) | Zanichelli | 2019 | 978-88-08-52055-5 |
Examination Methods
Oral Exam
