Teaching code

4S01530

Teacher

Carlo Morandi

Coordinator

Carlo Morandi

Credits

8

Language

Italian

Scientific Disciplinary Sector (SSD)

BIO/13 - EXPERIMENTAL BIOLOGY

Period

II° SEMESTRE dal Feb 13, 2023 al May 26, 2023.

Lessons timetable Moodle Seminars 0

Learning objectives

To offer the basic knowledge of human biology in an evolutionary perspective, underlying the molecular and cellular processes shared by all living organisms. To encourage students to critically evaluate experimental data by illustrating prime experiments of the past and contemporary biology. To describe the following processes: duplication, transmission, expression of the hereditary information; how changes arise To describe the hierarchy of master genes involved in tooth development and their interactions. To offer an updated information about the recombinant DNA technology and its applications to dentistry To teach the genetic bases of inherited diseases and how to interpret their modes of transmission To illustrate in particular various genetic conditions affecting dental health. At the end of the course, students must demonstrate to have gotten acquainted with basic knowledge of cellular functions, cell reproduction, cell-cell interactions, organism-to –organism interactions and organisms-to-environment interactions. They must also demonstrate to know how genetic traits are transmitted (mendelian and post-mendelian genetics, population genetics). They should be able to recognize inheritance patterns of genetic disorders, in particular those involving teeth development and structure. All these notions are a pre-requisite for further in depth studies , which will be undertaken by the students in subsequent courses.

Prerequisites and basic notions

no prerequisites are required

Program

MODULE OF BIOLOGY
Introduction to Biology. Diversity and universal features of living organisms. Evolution. The origin of life on earth. The scientific method. Model organisms.
The cell. Cellular theory. Cells as fundamental unit of living organisms. Fundamental properties of cells. Cell organization.
Biomolecules. The chemistry of life. Lipids. Polysaccharides. Proteins. Nucleic acids.
Structure and function of DNA. DNA replication. Replication of telomeres and telomerase. Repair systems of DNA damage. Analysis of DNA: molecular hybridization, Polymerase Chain Reaction (PCR).
From DNA to proteins: gene expression. Transcription. Maturation of mRNA. Translation and the genetic code.
Regulation of gene expression. Regulation of gene expression in prokaryotes. Regulation of gene expression in eukaryotes. Positive and negative regulation of transcription. Epigenetic modification. Gene expression in development. Virus gene expression.
Signal transduction. General concepts. Signal molecules and receptors. Signaling pathways. Cellular responses to signals.
Cell division and the cell cycle. The cell cycle and its regulation. Mitosis. Meiosis.
Cell death. Apoptosis and necrosis. Apoptotic signals. Caspases. Apoptosis pathways. BCL2 proteins.
Gene mutation. Types of mutations. Causes of mutations. Mechanisms of DNA repair.
Cancer. The hallmarks of cancer. Critical genes in cancer.
Molecular medicine.
MODULE OF GENETICS
Organization of the human genome. Characteristics of nuclear genome: single-copy coding sequences, highly and moderately repetitive DNA sequences. Gene distribution on chromosomes. Examples of gene families. Transposons and genomic changes. Comparative genomics. The mitochondrial genome: peculiar features.
Normal and pathological human karyotype. Methods of prenatal and postnatal analysis. Cytogenetic anomalies and syndromes.
Chromosomal mechanism of sex determination. The role of SRY and DAX1. Inactivation of the X chromosome and dosage compensation.
Mendelian Genetics. The laws of the transmission of characters, terminology in Genetics. Morgan’s experiments, gene association and recombination.
Human Genetics. Determination of blood groups: ABO, Rh. Pedigrees. Autosomal dominant or recessive characters, sex-linked inheritance.
Medical Genetics. Examples of autosomal dominant, recessive, X-linked diseases.
Examples of Mendelian disorders in Dentistry. Amelogenesis imperfecta, dentinogenesis imperfecta.
Molecular Genetics in Dentistry. Master gene regulators involved in tooth development, signaling pathways and their disfunctions. Examples of related syndromes. Dental agenesis.
Exceptions to Mendelism. Imprinting, uniparental dysomy, mithocondrial inheritance, dynamic mutations.
Multifactorial characters and diseases. Examples (including dental caries) and study approaches.
Population Genetics. Changes of population genotypic frequencies, the Hardy Weinberg law, disturbing factors. Effects of selection.
DIDACTIC MODES
Attendance to lessons is mandatory. Classes will consist of theorical lessons covering the whole exam program. Oral explanations will be coadiuvated by PowerPoint presentations and videos, which will be made available to students through a dedicated Department web site. Additional didactic supports (multiple choice quizzes for self-assessment, journal articles, reviews, etc.) may be suggested during the course and will be made available to students for download. During the whole Academic Year, students may request personal reception to the teachers, by e mail.

Bibliography

Didactic methods

Frontal lesson

Learning assessment procedures

The exam consists of a written test held simultaneously for the Biology and Genetics modules and potentially covers all the topics listed in the syllabus. For each module the questions are structured in 13 multiple-choice quizzes and 2 open-ended questions. Objectives of the written test are: a) assessment of the acquisition of theoretical knowledge; b) assessment of reasoning ability and individual re-elaboration of the notions acquired; c) ability to apply what has been learned to the resolution of experimental questions or to the interpretation of photographic images.

Evaluation criteria

The score of the written tests (Biology and Genetics) - awarded out of thirty - is decisive for the final grade. The written test is considered sufficient if the student correctly answers 8 multiple choice quizzes and at least 1 open question. Only in the event of a positive vote in the written tests, an oral interview based on the deepening of some topics is foreseen, for the purpose of an overall evaluation. The student can withdraw at any stage of the exam (written and oral), thus canceling any partial credit. The exam is carried out face-to-face.

Exam language

italiano