Studying at the University of Verona
Here you can find information on the organisational aspects of the Programme, lecture timetables, learning activities and useful contact details for your time at the University, from enrolment to graduation.
Study Plan
This information is intended exclusively for students already enrolled in this course.If you are a new student interested in enrolling, you can find information about the course of study on the course page:
Laurea in Bioinformatica - Enrollment from 2025/2026The Study Plan includes all modules, teaching and learning activities that each student will need to undertake during their time at the University.
Please select your Study Plan based on your enrollment year.
1° Year
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2° Year activated in the A.Y. 2015/2016
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3° Year activated in the A.Y. 2016/2017
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Un insegnamento a sceltaDue insegnamenti a scelta| Modules | Credits | TAF | SSD |
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Un insegnamento a sceltaDue insegnamenti a sceltaLegend | Type of training activity (TTA)
TAF (Type of Educational Activity) All courses and activities are classified into different types of educational activities, indicated by a letter.
Molecular biology laboratory (2016/2017)
Teaching code
4S01911
Credits
6
Language
Italian
Scientific Disciplinary Sector (SSD)
BIO/11 - MOLECULAR BIOLOGY
The teaching is organized as follows:
Laboratorio [II turno]
Laboratorio [I turno]
Teoria
Learning outcomes
The primary educational aim of the course is to provide the student an overview on the most common methodologies and techniques for the manipulation of DNA. In particular, the course covers the main techniques for purification of nucleic acids, their separation by electrophoresis, DNA amplification and methods for cloning in bacterial vectors.
In the practical exercises the student will apply these techniques for purification of plasmid DNA and genomic DNA, separation of DNA by electrophoresis on agarose gel, its digestion with restriction enzymes, preparation of gene constructs and their transformation into E. coli.
At the end of the course, the student will have learned the basics of manipulation of genetic material and will be able to use the main techniques for gene cloning.
Program
Theory:
The recombinant DNA. Cloning of Genes.
Bacterial and non bacterial vectors. cDNA and genomic libraries.
Manipulation of nucleic acids. Digestion with restriction endonucleases. Electrophoresis. Ligation of the DNA.
Polymerase Chain Reaction (PCR) and its applications.
Bacteria colutres. Trasformation of bacterial and eukaryotic cells.
Production of recombinant proteins.
Laboratory experiences:
Purification of plasmid DNA
Purification of genomic DNA
Purification of RNA
Electrophoresis and purification of DNA from gel.
Digestion with restriction enzymes.
PCR and PCR product purification.
Transformation, colony PCR
Expression of a recombinant protein and its analysis by SDS PAGE and Western Blot.
Bibliography
| Activity | Author | Title | Publishing house | Year | ISBN | Notes |
|---|---|---|---|---|---|---|
| Laboratorio | Amaldi, Benedetti, Pesole, Plevani | Biologia Molecolare (Edizione 3) | Ambrosiana | 2018 | 978-88-08-18518-1 | |
| Teoria | Karcher | LABORATORIO DI BIOLOGIA MOLECOLARE | ed Zanichelli | 1998 | ||
| Teoria | Michael R. Green e Joseph Sambrook | Molecular Cloning: A Laboratory Manual, Fourth Edition (Edizione 4) | CSHL Press | 2012 | 978-1-936113-42-2 |
Examination Methods
Laboratory activities report and oral examination
