Teaching code

4S000351

Credits

8

Coordinatore

Martina Montagnana

Language

Italian

Moodle Seminars

The teaching is organized as follows:

Learning outcomes

MODULO BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
The course aims to provide students with the principles for knowledge:
of specific terminology; direct and indirect analysis techniques for air, water and soil pollutants; the mechanisms, causes, effects and costs of pollution; purification techniques and self-purification phenomena; of techniques to limit pollution and its effects.

MODULO BIOLOGIA MOLECOLARE
The course also aims to provide an updated view of molecular biology techniques that are used in different clinical settings including prenatal diagnosis of genetic and chromosomal disorders, diagnosis of mendelian and oncoematologic diseases, infertility and pharmacogenetics.

MODULO BIOTECNOLOGIE RICOMBINANTI
The course aims to make students understand the basic stages of the birth of modern biotechnologies and the current applications of recombinant biotechnology in biomedical and pharmaceutical fields. We will discuss issues of a scientific, ethical and biological nature. The course also aims to provide an updated view of recombinant techniques including techniques for reproductive and therapeutic cloning, technic for the reproduction of recombinant drugs

MODULO METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
The training objective is to provide adequate knowledge of the theoretical principles and application of the techniques for detection of genetic alterations, in a functional framework considering the main diseases related to alterations of genes, including cancer.

MODULO METODOLOGIA DIAGNOSTICA MOLECOLARE
The course aims to make students understand the role of genetic testing in the clinical course of the patient. The course also aims to provide students who have no medical training the basic knowledge on the clinical aspects concerning the genetic basis of diseases. The course aims to provide an updated view of molecular biology techniques that are used in a clinical setting.

Program

MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE

MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
• Introduction and organization of the molecular biology laboratory • Extraction of nucleic acids • Electrophoresis of nucleic acids • Hybridization dot/blot and reverse dot/blot • Clinical molecular biology in hematological field • CBC and peripheral blood smear • Main methods of proteomics (chromatography, spettrometry..) • FISH and Array-CGH

MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE


MM: BIOTECNOLOGIE RICOMBINANTI
1) History of modern Biotecnology. The Reproductive cloning. The Therapeutic cloning. Critical review of two fundamental scientific works: 1) Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH.Viable offspring derived from fetal and adult mammalian cells.Nature. 1997 Feb 27;385(6619):810-3. 2) Schwartz SD, Regillo CD, Lam BL, Eliott D, Rosenfeld PJ, Gregori NZ, Hubschman JP, Davis JL, Heilwell G, Spirn M, Maguire J, Gay R, Bateman J, Ostrick RM5, Morris D, Vincent M, Anglade E, Del Priore LV, Lanza R. Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: follow-up of two open-label phase 1/2 studies. Lancet. 2015 Feb 7;385(9967):509-16. 2) Cloning vectors. Role of DNA ligase restriction enzymes, alkaline phosphatase. Plasmids. Methods for introducing DNA into a host cell: transformation and transfection. Vectors selection and isolation. 3) Transgenic animals. Gene transfer techniques: irestriction-enzyme-mediated integration (REMI), DNA microinjection, Sperm-mediated gene transfer, Retrovirus-mediated gene transfer. Gene targeting. knockout mice. 4) Clinical applications of recombinant drugs. Human recombinant TSH. production technique, commercial products, pharmacokinetic properties, approval for clinical use. Use in the diagnosis and follow-up of differentiated thyroid carcinoma 5) Clinical applications of recombinant drugs. Recombinant erythropoietin: production technique, commercial products, pharmacokinetic properties, approval for clinical use. Recombinant insulin: production technique, pharmacokinetic properties, clinical use, commercial products.

MM: BIOLOGIA MOLECOLARE
•Introduction and organization of the molecular biology laboratory •Cystic fibrosis: pathogenesis, clinical manifestations and diagnosis •Male infertility: laboratory tests in the diagnosis of congenital causes •Molecular biology in oncohematological diseases •Prenatal diagnosis: molecular and cytogenetic testing •Diagnostics of Genetic diseases in mental retardation •Hemocromatosis: pathogenesis, clinical manifestations and diagnosis •Application of Pharmaco-genetics and -genomics

Bibliography

Examination Methods

MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
Oral exam

MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
Written test, with open questions and multi choice questions. The exam is based on all the course contents. The exam is passed if the evaluation is greater or equal to 18/30.

MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
Oral Exam

MM: BIOTECNOLOGIE RICOMBINANTI
Oral exam

MM: BIOLOGIA MOLECOLARE
Oral exam